海马微量注射β淀粉样蛋白对Bax/Bcl-2表达的影响

The effect of β-amyloid protein microinjected into hippocampus on expression of Bax and Bcl-2

目的 探讨Bax/Bcl -2基因表达在 β -淀粉样蛋白 ( β -amyloidprotein ,Aβ)脑内致凋亡机制中的作用。 方法 用微量注射器将Aβ 1-4 0注射到大鼠右侧海马CA1区诱发Aβ在脑内该区域的沉积 ,7d后 ,用免疫组化SABC法检测Bax/Bcl -2的表达。结果 同生理盐水对照组及假手术对照组相比 ,Aβ组Bax表达增强 ,而Bcl-2表达在 3组间差异无显著性。结论 Aβ脑内沉积诱导神经元凋亡的机制可能与其增强了Bax的表达有关。

Objective To investigate the role of Bax and Bcl-2 in apoptosis induced by β-amyloid protein in the brain.Methods Aβ 1-40 was injected into CA1 subfield of rat's right hippocampus. The expression of Bax and Bcl-2 was determined with immunohistochemistry SABC method seven days later.Results The expression of Bax in hippocampal CA1 subfield in Aβ group was stronger than that in the sham operation and saline control group, but the expression of Bcl-2 was not significantly different among the three groups.Conclusion Expression of Bax may play an important role in neuronal apoptosis induced by Aβ in the brain.