祛风通络方对肾小球系膜细胞周期蛋白表达的影响

Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells

目的通过不同检测方法观察祛风通络方对肾小球系膜细胞周期调节蛋白表达的影响,进一步探讨祛风通络方抑制肾小球系膜细胞增殖的作用机制。方法以体外培养肾小球系膜细胞为研究对象,应用血清药理学方法,分别采用激光扫描共聚焦显微镜及免疫组化法检测各组大鼠肾小球系膜细胞周期蛋白的表达变化。结果与正常组比较,LPS组肾小球系膜细胞周期蛋白CyclinD1、CDK2、P21表达明显增强(P<0.01),P27蛋白表达明显减弱(P<0.01);经过药物干预治疗,肾小球系膜细胞周期蛋白Cyclin D1、CDK2、P21的表达均有不同程度减弱(P<0.05或P<0.01),而P27蛋白表达明显增强(P<0.01),且祛风通络方对Cyclin D1、P21和P27蛋白表达的调节明显优于贝那普利(P<0.05或P<0.01)。结论祛风通络方抑制肾小球系膜细胞增殖的作用机制可能与其下调细胞周期蛋白Cyclin D1、CDK2、P21和上调P27蛋白的表达有密切关系。

Objective To observe the effects of Qufengtongluo Redpe （QFTLR） on the expressions of cell cycle regulatory proteins in rat mesangial cells （MCs） in vitro and investigate the mechanism by which QFTLR inhibits MC proliferation. Methods Using the methods of serum pharmacology, we studied the expressions of cell cycle regulatory proteins in rat MCs treated with QFTLR by laser scanning confocal microscope and immunohistochemistry. Results Compared with the normal control cells, the cells challenged with lipopolysaccharide （LPS） showed significantly enhanced expressions of cyclin D1, CDK2, and P21 （P〈0.01） and obviously lowered protein expression of P27 （P〈0.01）. Treatment of the LPS-challenged cells with QFTLR and benazepril both resulted in significantly attenuated expressions of cyclin D1, CDK2, and P21 and obvious increase of P27 expression （P〈0.05 or P〈0.01）, but QFTLR produced stronger effects than benazepril in regulating of cyclinD1, P21 and P27 protein expressions （P〈0.05 or P〈0.01）. Conclusion QFTLR inhibits rat MC proliferation in vitro possibly by down-regulating the cellular expressions of cyclin D1, CDK2, and P21 and up-regulating the expression of P27 protein.