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中国人IL-18结合蛋白cDNA的克隆和序列分析 被引量：7

Cloning and sequencing of human IL-18 binding protein cDNA
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摘要 目的克隆人白介素 - 18结合蛋白 (h IL- 18BP)的 c DNA,以研究其结构与功能的关系。方法从中国汉族人的胎盘组织中提取总 RNA,以 RT- PCR方法扩增出全长 5 85个 bp的 IL- 18BP c DNA,将其与表达载体 pc DNA3连接 ,转化大肠杆菌 DH5 α,建立了 h IL- 18BP的 c DNA克隆。结果序列分析表明 ,与国外文献报道的人 IL- 18BP的 c DNA序列完全一致。结论 h IL- 18BP已成功地得到克隆。 ObjectiveTo obtain the cDNA of human interleukin 18(IL 18) binding protein(hIL 18BP) and to investigate the relationship between structure and function of hIL 18BP.MethodsThe total RNA was extracted from human placenta and the intact cDNA of hIL 18BP was amplified by RT PCR.The cDNA was cloned into pcDNA3 vector and transformed into E.coli DH5α.Results The result of sequencing was completely consistent with the published hIL 18BP cDNA.Conclusion hIL 18BP cDNA has been cloned successfully. [
作者 傅奕 赵惠仁 裴冬生
机构地区 徐州医学院生物化学与分子生物学研究中心
出处 《免疫学杂志》 CAS CSCD 北大核心 2000年第4期254-256,共3页 Immunological Journal
关键词 白细胞介素-18结构蛋白 CDNA 克隆 序列分析 interleukin 18 binding protein RT PCR cDNA cloning DNA sequencing
分类号 R392.11 [医药卫生—免疫学]
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参考文献9

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同被引文献76

  • 1冷建杭 张立煌 姚航平 等.表达IL-18重组腺病毒对小鼠腹腔巨噬细胞免疫效应功能的影响[J].中华微生物学和免疫学杂志,2001,21(1):29-29.
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引证文献7

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免疫学杂志
2000年 第4期

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