抗CD34单抗标记血管生成在非小细胞肺癌中的表达及临床意义

Expression and clinical significance of angiogenesis labelled by anti CD34 monoclonal antibody in non small cell lung carcinoma

目的探讨非小细胞肺癌中 CD34标记的血管生成的表达及临床意义。方法采用免疫组化法检测 81例手术切除的非小细胞肺癌标本中抗内皮细胞表面抗原 CD34单抗标记的血管生成。结果 CD34以其染色的特异性、敏感性及清晰的背景而易于识别。统计学分析显示微血管密度同肺癌临床分期的进展及血行转移密切相关 ,但未发现同淋巴结转移及其它临床病理特征有关。结论肺癌中微血管密度对血行转移的进展有重要作用且同肺癌的发生有关。

ObjectiveTo study expression and clinical significance of angiogenesis labelled by anti CD34 monoclonal antibody in non small cell lung carcinoma.Methods Non small cell lung carcinoma tissue were obtained postoperatively from 81 patients with lung carcinoma.Immunohistochemical detection of tumor associated microvessels was performed using anti CD34 monoclonal antibodies directed against endothelial markers.Results Staining of CD34 was easy to identify because of its specificity,sensitivity and clear background.Statistic analysis showed:the microvessel count was significantly related to the development of disease and hematogenous metastasis but did not correlate with the development of nodal metastasis and other clinicopathologic characteristics.Conclusion Tumor vascularity is important for the development of hematogenous metastasis and correlates with lung carcinogenesis. [