抗HCMV不同多肽McAb的鉴定

Identification of McAb against different HCMV polypeptides

目的为了将单克隆抗体 (Mc Ab)用于诊断技术 ,我们首先建立了 13株抗人巨细胞病毒 (HCMV)的 Mc Ab,选取 6株 Mc Ab作进一步鉴定。方法这 6株 Mc Ab的鉴定主要采用间接免疫荧光试验、免疫印迹试验等方法。结果间接免疫荧光试验表明 :Mc Ab8B8相应的多肽为早期抗原 ;而 Mc Ab7B4、7D7、7E7、7E11、8D6的相应病毒多肽为晚期抗原。免疫印迹试验的结果表明 :Mc Ab7B4、7D7、7E7、7E11、8B8、8D6相应的病毒多肽分子量分别为 46、15 0、38、5 1、72、6 5 kd。结论 Mc Ab8B8可不用于 HCMV的快速诊断。

ObjectiveTo apply monoclonal antibody(McAb) to diagnostic techniques,13 McAbs against different human cytomegalovirus(HCMV) polypeptides were established and 6 McAbs(7B4,7D7,7E7,7E11,8B8,8D6) were selected for further identification Methods 6 McAbs were mainly identified by indirect immunofluorescence reaction and immunoblot analysis ResultsIndirect immunofluorescence reaction of 6 McAbs with HCMV infected cells at different time showed that McAb 8B8 is against the early antigen of HCMV,while McAbs 7B4,7D7,7E7,7E11,8D6 are against the late antigen of HCMV Immunoblot analysis revealed 6 McAbs are directed against different HCMV polypeptides with molecular weight of approximately 46,150,38,51,72,65 kd.ConclusionMcAb 8B8 can be used for rapid assay of HCMV. [