血清EB病毒特异性DNA酶抗体的检测

Detection of sera Epstein-Barr virus-specific DNase antibody

目的 建立一种新的鼻咽癌 (NPC)血清学诊断方法。方法 以纯化的EBV -DNase为抗原 ,采用WesternBlot法对NPC、头颈部其它肿瘤 (OT)及健康成人 (HA)血清中的EBV -DNase抗体进行检测。与同时进行的EBVCA -IgA抗体检测比较。 结果 ①用WesternBlot法检测NPC组、OT组、HA组血清中EBV -DNase抗体阳性率分别为 83.71% ,0 .0 0 % ,0 .0 0 % ,NPC组抗体阳性率与其它两组差异显著 (均为P <0 .0 1) ,后两组之间无显著差异 (P >0 .0 5)。而同时检测的EBVCA -IgA阳性率分别为85.98% ,9.38% ,7.50 %。两种方法阳性率比较 ,在NPC组无差异 (P >0 .0 5) ,而在其它两组的差异显著 (P <0 .0 5) ;②以病检结果为“金标准” ,EBV -DNase抗体WesternBlot法检测的特异性明显高于EBVCA-IgA检测 (P <0 .0 1) ,而两者的灵敏度相当 (P >0 .0 5)。结论 ①NPC血清中存在高滴度的EBV -DNase抗体 ;②用WesternBlot法检测血清中的EBV -DNase抗体 ,具有较高的灵敏度及特异性 ,可以减少NPC的漏诊和误诊 。

Objective To establish a new seriological diagnostic method for nasopharyngeal carcinoma (NPC). Methods With purified EBV-DNase as antigen, sera EBV-DNase antibody was determined by Western Blot. With Western Blot, sera from NPC patients, patients with other head and neck tumors (OT) and healthy adults (HA) were assayed. All the sera undertook EBVCA-IgA examination. Results ①The EBV-DNase antidody positive rates of the NPC group, OT group and HA group determined by Western Blot were 83.71% , 0.00% and 0.00% . The differences of the EBV-DNase antibody positive rates between the NPC and the other two groups were high statistically significant (P< 0.01 ). The difference between the OT and HA was insignificant (P> 0.05 ). The EBVCA-IgA positive rates of the three groups were 85.98% , 9.38% and 7.50% . Comparison of the antibody positive rates between EBV-DNase and EBVCA-IgA showed that their differences in the NPC group were insignificant (P> 0.05 ), and those in the other two groups were significant (P< 0.01 , P< 0.01 ); ②The specificity of EBV-DNase determination by Western Blot was much higher than that of EBVCA-IgA examination (P< 0.01 ) while their sensitivities were almost equal (P> 0.05 ). Conclusion ①There is a high antibody titer to EBV-DNase in NPC sera. ②Detection of sera EBV-DNase antibody by Western Blot has a high specificity and sensitivity. Its clinical application may reduce the misdiagnosis and failure in the diagnosis of NPC. It may be an effective seriological method for the diagnosis of NPC.