水杨酸对白蜡属种间杂交子代悬浮细胞中香豆素类含量的影响

The Effect of Salicylic Acid on Coumarin Content in Fraxinus Hybrid Cell

为了研究不同浓度水杨酸(SA)对白蜡属种间杂交子代悬浮细胞生长量及香豆素类物质产量的影响,从而建立以生产香豆素类物质为目的的白蜡属细胞悬浮培养技术。以白蜡种间杂交子代悬浮培养96h的细胞为材料,分别添加不同质量浓度(25、50、100mg/L)的SA处理8、24、48、72、96h,进行细胞生长量、香豆素类化合物类含量测定。结果表明,施加信号分子SA能不同程度地促进香豆素及白蜡树精的含量和产量增加,其中以浓度100mg/L的SA效果最明显,处理72h后白蜡树精的含量及产量均达到最高,分别为0.5704mg/g和0.2595mg,相较于对照分别提高了114.59%和35.03%,香豆素的含量和产量的变化也有相同的趋势,均在处理72h后达到最高,分别为0.2470mg/g和0.1124mg,相较于对照分别提高了112.39%和56.56%。适宜浓度的SA可促进白蜡属种间杂交子代悬浮细胞中香豆素类物质的积累。

In order to study the effects of SA on the contents of coumarins in the suspension cultured cells of Fraxinus hybrid which paved the way for establishing plant cell suspension cultures of Fraxinus hybrid,three different concentration of SA（25,50,100mg/L） were applied in the suspension cultured cells which had been cultured for 96h.Then the cell growth and content of coumarins were determined at 8h,24h,48h,72h and 96h after treatment.The results showed that the signal molecule SA raised the contents and productions of coumarin and fraxinol to different respectively.Among the three concentrations,100mg/L of SA brought the best effect to coumarins accumulation.The content and production of fraxinol in the suspension cultured cells which was harvested at 72h after treatment were 0.5704mg/g and 0.2595mg which were 114.59% and 35.03% higher than control,respectively.The variation trends of the content and production of coumarin were the same.They reached the maximum at 72h after treatment and were 0.2470mg/g and 0.1124mg which were 112.39% and 56.56% higher than the control.It concluded that the appropriate concentrations of SA could effectively promote the accumulation of coumarins in Fraxinus interspecific hybrid suspension cell.