大鼠肠道缺血再灌注损伤对肺磷脂的影响

Effects of intestinal ischemia-reperfusion injury on pulmonary phospholipid in rats

目的 观察肠道缺血再灌注 (IIR)损伤对肺磷脂的影响。方法 制作IIR大鼠模型 ;肺磷脂组份采用毛细管电泳法分析。结果 肠道缺血 6 0min再灌注可引起肺磷脂组份的明显改变。PC和PE在再灌注后出现降低 ,其中PC降低明显 ,于 72h较缺血前降低 35 .1% ;而PI于再灌注后 48h、PS在 2 4h分别增加2 9.0 %和 37.9% ;SM在再灌注后 6h增加达峰值后下降 ,至 72h接近缺血前水平 ;其它磷脂在再灌注后明显升高 ,较缺血前增加 1倍以上。相关分析提示PC的改变与其他组份无明显相关性 ,PE、PS的变化则与SM呈显著负相关。结论 肠道IIR可致肺磷脂组份的明显改变 ,可能是肠道IIR后肺损伤的重要机制之一。

Objective To observe the changes of the components of pulmonary phospholipid after intestinal ischemia reperfusion injury (IIR) in rats. Methods Rat model of intestinal ischemia reperfusion injury was established. The components of pul monary phospholipid was determined with micellar electrokinetic capillary chromatography (MECC). Results Significant changes of percentages of pul monary phospholipd components occurred after IIR injury.Among them, phosphatidylcholin and phosphatidylethanolamine were decreased in the period of reperfusion and phosphatidylcholin was 35.1% lower than its preischemic level 72 hours after ischemia. Phosphatidylinoside was increased by 29.0% in the 48th hour and phosphatidylserine was increased by 37.9% in the 24th hour after ischemia. Sphingomylin was increased to the peak in the 6th hour after ischemia and then declined and restored its preischemic level 72 hours after ischemia. Other components of phospholipid was increased nearly by twofold after reperfusion as compared with the preischemic level. Correlation analysis showed that the changes of phosphatidylcholin showed no correlation to other components and those of phosphatidylethanolamine and phosphatidylserine were negatively correlated to those of sphingomylin. Conclusion The percentages of the components of pulmonary phospholipid can be changed with IIR injury, which may play an important role in the pathological mechanism of acute lung injury.