^(32)P标记寡聚核苷酸探针检测胃癌组织中MUC5AC mRNA表达

Expression of MUC5AC mRNA in the tissuses of gastric carcinoma with ^(32)P-labeled oligonucleotide probe

目的 揭示正常胃粘膜、癌前病变和胃癌组织中MUC5AC基因转录水平的表达及其临床病理意义。方法 应用32 P标记寡聚核苷酸探针和原位杂交方法检测组织切片中的MUC5ACmRNA的表达。结果 人正常胃粘膜中的浅表 1/3范围内广泛分布MUC5AC基因的mRNA ,肠上皮化生和胃癌组织中的表达阳性率分别是 15 .4%和 2 5 %。胃癌组织中MUC5ACmRNA的表达与胃癌患者的性别、肿瘤的部位和大小、淋巴结转移、肿瘤的分化和浸润、临床分期无关 (P >0 .0 5 ) ,但MUC5AC阳性组和阴性组之间的胃癌Lauren’氏分型差别显著 (P <0 .0 5 )。结论 MUC5ACmRNA在胃癌和肠上皮化生组织中呈下调表达 。

Objective To explore the expression of transcriptional level of mucin 5AC (MUC5AC) gene in the normal gastric mucosa, precancerous lesion and gastric carcinoma and its significance. Methods The expression of MUC5AC mRNA in tissue slices was detected with 32 P labelled oligonucleotide probe and in situ hybridization. Results MUC5AC mRNA was expressed extensively in one-third of the superficial normal gastric mucosa and the positive rate of the expression was 15.4% in intestinal metaplasia and 25% in gastric carcinoma. The expression of MUC5AC mRNA in gastric carcinoma was not correlative with sex and clinical stage of the patients, metastases of lymph nodes, and the size, location, infiltration and differentiation of the cancer ( P >0.05). There was a significant difference of Lauren's classification of gastric cancers between the MUC5AC positive and negative groups ( P < 0.05). Conclusion The expression of MUC5AC mRNA in intestinal metaplasia and gastric carcinoma was decreased and the expression in gastric carcinoma is related to Lauren's classification.