摘要
目的:探讨微小RNA let-7i在胃癌中的表达及其与临床病理特征的关系。方法:用茎环RT-PCR的方法检测68例胃癌患者的肿瘤组织及癌旁正常组织中let-7i的表达,并分析其与胃癌临床病理指标及化疗敏感性的关系。结果:胃癌中let-7i的表达明显低于配对的癌旁正常组织(P<0.001),且let-7i的异常表达与肿瘤的局部侵袭(P<0.001)、淋巴结转移(P<0.006)、分化程度(P<0.018)及化疗抵抗(P<0.001)显著相关。结论:miRNA let-7i可能是一个潜在的生物标志物和分子靶标,可在胃癌的早期诊断和基因治疗中发挥作用。
Objective： To investigate the expression of miRNA let-7i in gastric cancer and its relationship with clinicopathologic features. Methods： The expression of iet-7i in 68 pairs of primary gastric cancer and normal adjacent tissues （NATs） was quantified by stem-loop RT-PCR, and the relationship between let-Ti level and clinicopathologic features and chemosensitivity was explored. Results： Let-7i was significantly down-regulated in cancer tissues compared with the paired NATs （P 〈 0.001 ）. Low expression of let-Ti showed significant correlation with local invasion （P 〈 0.001）, lymphatic metastasis （P = 0.006）, poor differentiation （P = 0.018）, and resistance to chemotherapy （P 〈 0.001）. Conclusion： These findings indicate that let-7i may be a potential biomarker and molecular target for early diagnosis and gene therapy in gastric cancer.
出处
《临床药物治疗杂志》
2012年第6期12-15,共4页
Clinical Medication Journal
