牛磺酸对肾性高血压大鼠心肌血管紧张素Ⅱ及胶原含量的影响

The Effect of Taurine on Myocardial Local Angiotensin Ⅱ and Collagen Concentration in Renovascular Hypertensive Rats

目的 :观察牛磺酸 (Tau)对肾血管性高血压大鼠心肌血管紧张素Ⅱ (AngⅡ )及胶原含量的影响 ,并探讨左室肥厚的机制。方法 :建立二肾一夹 ( 2K1C)肾血管性高血压大鼠模型。应用放免法测定AngⅡ (MAC) ,羟脯氨酸法测定大鼠心肌胶原含量 (MCC)。显微镜下观察心肌组织结构改变 ,测微计测量心肌纤维直径 (MFD) ,并对MCC及MFD与MAC进行线性相关分析。结果 :2K1C肾性高血压大鼠MCC及MFD与MAC含量较假手术组 (Sham组 )显著增加 (P <0 .0 1 )。MCC及MFD与MAC显著正相关 (r =0 .83 2 3 ,0 .83 51 3 ,P <0 .0 1 )。牛磺酸可显著降低 2K1C大鼠尾动脉收缩压 (SBP)、MFD、MAC、MCC和左心室 /体重比值 (LVW /BW) ,并抑制心肌细胞肥大及心肌间质的增生。结论 :2K1C大鼠心肌肥厚与心肌局部AngⅡ增加有关 ;牛磺酸可能通过降低心肌AngⅡ含量而逆转肾性高血压左室肥厚。

Objective To study the protective effect of taurine on myocardial local angiotensin Ⅱ(Ang Ⅱ)and collagen concentration in renovascular hypertensive rats,and to research the mechanism of left ventricle hypertrophy.Methods Two kidney 1 clip (2K1C) rats were used to reestablish the model of renovascular hypertension.The myo cardial local Ang Ⅱconcentration (MAC) was measured by radioimmunoassay.The myocardial collagen concentration (MCC) was measured by biochemical method.The myocardial tissue structure was observed under the microscope,and the myocardial fiber diamension (MFD) was measured with micrometer.The linear correlation between MCC,MFD and MAC were analysed.Results Compared with sham operated rats,the MCC and MFD were significantly increased in 2K1C rats (P&lt;0.01),both of which positively correlated with MAC(r=0.8323,0.83513,P&lt;0.01).Taurine have an obvious suppressive effect on SBP,LVW/BW,MAC,MCC, MFD(P&lt;0.001 for all these indices), myocardial cell hypertrophy and myocardial collagenous fiber productive reaction in 2K1C rats.Conclusion The myocardial local Ang Ⅱ may play roles in the myocardial hypertrophy in 2K1C rats .Taurine can produce good protective effect on left ventricle hypertrophy probably by decreasing the myocardial local Ang Ⅱ concentration.