川芎嗪对大鼠脑缺血再灌注损伤的保护作用

Protective Effects of Tetramethylphrazim on Cerebral-Ischemia Reperfusion Damages in Rats

目的 :观察大鼠海马CA— 1区缺血再灌注损伤后MDA值和ATPase活性的动态变化及川芎嗪的保护作用。方法 :Wistar大鼠双侧颈总动脉夹闭 2 0min ,造成不完全性脑缺血 ,松夹即为再灌注。大鼠随机分为假手术对照组 (SOC)、缺血再灌注组 (IR)和川芎嗪治疗组 (TMP)。结果 :①IR组MDA值再灌注 6h后恢复对照水平 ,川芎嗪能显著降低异常增高的MDA值 (P <0 .0 1 )。②IR组ATPase活性再灌注 2 4h后恢复对照水平 ,川芎嗪能明显改善酶活性 (P <0 .0 1 ) ,且能缩短酶活性恢复时程。结论 :川芎嗪能减少大鼠脂质过氧化 ,改善ATPase功能 ,减轻海马结构缺血再灌注损伤。

Objective To observe the dynamic changes of malondialehyde(MDA) content and ATPase activity in the hippocampus structure in rat suffering from ischemia reperfusion damage,and to investigate the protective effects of tetramethylphrazim(TMP).Methods An cerebral ischemia model of Wistar rats was getten by clipping bilatral carotide,and reperfusion was performed when the clips were released.Rats were randomly divided into sham operated control group(SOC),ischemia reperfusion group(IR) and TMP treated group(TMP).Rusults ①MDA content came back to the control level 6 hours after reperfusion in IR,and TMP can lower MDA content obviously(P&lt;0.01);②ATPase activity came back to the control level 24 hours after reperfusion in IR.TMP can improve ATPase activity(P&lt;0.01) and shorten the period of the activity coming back.Conclusion TMP can reduce lipid peroxidation,improve ATPase activity and decrease neuronal damage in the IR injury of hippocampal structure.