武汉地区献血员血清TTV等几种肝炎相关病毒的基因检测

Detection of TTV DNA and Other Hepatitis Virus in Sera of Blood Donors in WUHAN Area

目的 :了解武汉地区不同献血员血清TTV感染特点及其与其它几种肝炎相关病毒感染之间的关系。方法 :采用PCR及巢式PCR对 54例武汉市城区义务献血员及 74例既往乡村献血者分别进行血清TTVDNA、HCVRNA及HGVRNA等检测。结果 :TTVDNA、HCVRNA及HGVRNA指标阳性率在既往乡村个体献血员为 4 3 .2 %、2 3 .0 %与 2 .7% ,城区义务献血员为 5.6%、1 .9%与 1 .9% ,前者TTVDNA与HCVRNA检出率较后者显著升高 (P <0 .0 1 ) ,两组TTVDNA的检出率显著高于其他肝炎相关病毒。结论 :武汉地区献血员人群存在TTV感染 ,长期的既往献血过程可使职业献血员TTV与HCV感染率升高 ;由此造成的TTV在部分乡村特殊人群中的流行已经成为武汉地区一个值得关注的流行病学问题。

Objective To investigate the infective characteristics of TTV and the relationship between it and otter hepatitis associate viruses infection in sera of blood donors in Wuhan area.Methods PCR or RT nested PCR were adopted to detection TTV DNA,HCV RNA and HGV RNA in sera of 54 volunteers and 74 professional blood donors.Results The detection rate of TTV DNA,HCV RNA and HGV RNA were 43.2%,23.0% and 2.7% in professional group and 5.6%,1.9% and 1.9% in voluntteer;the positive rate of TTV DNA and HCV RNA in the former group were obviously higher than in latter(P&lt;0.01).And in two groups,the detectioc rate of TTV DNA was significantly higher than the other hepatitis viruses.Conclusion In professional blood donors there is a high risk for TTV tansmission.