干细胞转录因子Nanog在肝细胞癌中的表达及意义

Significance of stem cell transcription factor Nanog expression in hepatocellular carcinoma

对2007年8月至2011年4月在我院行手术治疗的40例肝细胞癌（HCC）患者肝癌组织及其中10例患者的HCC癌旁组织、10例肝血管瘤的肝组织及5例精原细胞瘤组织，采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结法，行干细胞转录因子Nanog蛋白表达的检测。各类组织中Nanog蛋白表达的比例分别为42％（17／40）、1／10、0／10和5／5；Nanog蛋白表达与性别、年龄、血清AFP水平、HBsAg阳性、肿瘤分化情况、Child分级及TNM分期，无明显相关性（P〉0．05）。Nanog蛋白可作为HCC干细胞的表面标志物。

From August 2007 to April 2011, hepatocellular carcinoma （HCC） （n = 40）, para- HCC tissues （ n = 10）, seminoma （ n = 10） and cavernous hemangioma （ n = 10） were selected. And the method of immunohistochemical streptavidin-perosidase was applied to detect the protein expression of Nanog. The expression ratios of Nanog were 17/40 （42%）, 1/10, 0/10 and 5/5 in HCC, para-HCC tissues, seminoma and cavernous hemangioma respectively. Its expression showed no significant correlation with the patient gender, age, serum alpha fetoprotein （AFP）, hepatitis B surface antigen （HBsAg）, differentiation, Child grade and TNM stage （P 〉 0. 05 ）. It may be used as a surface marker of liver cancerstem cell.