腺苷对正常大鼠肺动脉压的影响及机制

The effect and mechanism of adenosine on pulmonary arterial pressure of normal rats

观察外源性腺苷对正常大鼠血流动力学的影响及可能的作用机制。方法 :从正常大鼠肺动脉内注入不同浓度的腺苷溶液或等量的生理盐水 ,持续 15分钟。结果 :2 5 μg/ (kg· min)组 (T组 )、5 0 μg/ (kg· min)组 (F组 )、10 0 μg/ (kg· m in)组 (H组 )大鼠用药后平均肺动脉压 (m PAP)分别增高 2 0 .6 %± 10 .0 %、33.8%± 18.7%、40 .4%± 17.6 % ,高于用药前 (P<0 .0 5、0 .0 5、0 .0 1) ,与生理盐水组 (S组 )比较 ,差异有显著性 (P<0 .0 5、0 .0 5、0 .0 1) ,T、F、H、S组用药前后平均体循环压 (m SAP)、TXB2 、6 - keto- PGF1 a、NO的水平及心率均无明显改变。结论 :腺苷引起正常大鼠肺动脉压剂量依赖性升高 ,其机制可能不通过环氧化酶代谢产物或 NO的途径。

Objective:To evaluate the effects and mechanism of adenosine on the systemic and pulmonary vessels of normal rats.Methods:Different concentrations of adenosine or an equal volume of saline was infused into the pulmonary artery of normal rats,which were divided into four groups.Each infusion maintained for 15min.Results:Adenosine in dose of 25、50、100μg/(kg·min) increased mPAP by 20.6%±10.0 %、33.8%±18.7% and 40.4%±16.7% respectively(P<0.05,0.05,0.01).The levels of TXB2, 6 keto PGF 1a,NO in 25 μg/(kg·min)(T group,n=5),50μg/(kg·min)(F group,n=5),100μg/(kg·min)(H group,n=5),saline(S group,n=5) have no significant changes.Conclusion:Adenosine increase the mPAP of the normal rats. It may not be mediated by the altering of cyclooxygenase products and NO.