霍奇金淋巴瘤EB病毒感染和p53蛋白表达的关系

The significance of Epstein Barr virus infection and expression of p53 protein in Hodgkins lymphoma

目的 :探讨EB病毒在霍奇金淋巴瘤 (HL)潜伏感染和 p5 3蛋白表达的关系。 方法 :应用EBER 1寡核苷酸探针以原位杂交 (ISH)方法检测 72例HL组织中的EBER ;用免疫组化方法检测 p5 3蛋白表达。 结果 :发现HL中R S细胞EBER 1和p5 3蛋白的阳性率分别为 5 3 2 0 %和 47 2 2 %。HL中EBER 1检出率明显高于淋巴结反应性增生组 (P <0 0 5 ) ,同时HL中的R S细胞含有EBV潜伏感染期中高拷贝 (RNA ,EBER 1)含量丰富 ,阳性信号在核仁蛋白膜表达。检测HL淋巴细胞衰减型 p5 3的阳性率较高。结论 :HL发生可能与EB病毒潜伏感染有关。EB病毒、p5 3之间可能存在内在联系并相互影响 ,p5 3蛋白阳性者预后不良。

Purpose To study the relationship between EBV infection of Hodgkins lyphoma(HL) and expression of p53 protein. Methods EBER and p53 protein expression in 72 cases of HL were studied with EBER 1 oligonucleotide probe and immunohistochemistry respectively.Results The rate of EBER positive R S cells and p53 expression in HL were 53 20% and 47 22%, the former was higher than that in group of lymph node hyperplasia(P<0 05). Meanwhile R S cell in HL contained a wealth of high copy EBER 1 in EBV latency. Conclusions HL might be related to EBV latent infection. Mutation of p53 gene may be related to the in fection of EBV and prognosis of patients who has p53 positive expression are poor.