肝癌细胞侵袭行为与其流变特性相关

Invasion Behavior of Hepatocellular Carcinoma Cells Correlates with Their Rheological Properties

以原代肝细胞、肝细胞癌（Hepatocellularcarcinoma，HCC）细胞及对人工基底膜Matrigel具有侵袭能力的HCC细胞（HCC-Inv细胞）为研究对象，研究了三种细胞的粘弹特性、与胶原蛋白Ⅰ的粘附力学特性及与肝血窦内皮细胞（Liversinosoidalendothelialcell，LEC）的粘附力学特性。结果发现，HCC细胞的上述流变特性较之于肝细胞发生明显的变化；与肝细胞及HCC细胞相比较，HCC－Inv细胞表现出高的粘弹特性，高的与胶原蛋白Ⅰ的粘附力，高的与LEC的粘附力；免疫细胞化学的结果证实了HCC细胞与HCC－Inv细胞形态与细胞骨架结构的差异。作者对肝癌细胞流变特性改变及病理学意义作了简要讨论。

We investigated the viscoelasticity,mechanics of adhesion to collagen Ⅰ coated surfaces and mechanics of adhesin to liver sinosoidal endothelial cells(LECs) among primary culture human hepatocytes,hepatocellular carcinoma(HCC) cells as well as HCC-Inv cells,namely HCC cells capable of invading the artificial basement membrane,Matrigel. The results showed that the above-mentioned rheological properties of HCC cells differed obviously from those of normal hepatocytes. HCC-Inv cells exhibited higher viscoelastic coefficients,higher adhesion forces to collagen Ⅰ coated surfaces as well as higher adhesion forces to LECs than those of normal hepatocytes and HCC cells. Immunocytochernistry showed the differences in cell morphology and cytoskeleton structure between HCC cells and HCC-Inv cells. A brief discussion was presented in relation to the changes in cell rheological properties of hepatocellular carcinoma cells and their pathological significance.