溶血磷脂酰胆碱对内皮细胞表面粘附分子表达的影响

Effect of Lysophosphatidylcholine on Endothelial Cell Surface Expression of Adhesion Molecules

大量的单核细胞募集是动脉粥样损伤形成的早期表现之一，相关的内皮细胞粘附分子在其中具有积极作用。本文研究了溶血磷脂酰胆碱（Lysophosphatidylcholine，Lyso－PC）对培养的人脐静脉内皮细胞（HUVECs）膜上细胞间粘附分子-1（Intercellularadhesionmolecule－1，ICAM－1）、E选择素（Endothelial－leukocyteadhesionmolecule－1，ELAM－1，E－selectin）和血管细胞粘附分子（Vascularcelladhesionmolecule－1，VCAM－1）表达的影响。用流式细胞仪分析Lyso－PC介导的粘附分子表达的变化。结果显示：与基础水平相比，用Lyso－PC处理后的HU－VECs表面ICAM－1和VCAM－1的表达增加，50μg／ml的Lyso－PC刺激1h后介导ICAM-1和VCAM－1最大表达，但3h后则下降到基础水平以下；而Lyso－PC对E－selectin的表达无显著影响，仅在50μg／ml的Lyso－PC刺激3h后开始显著上调。表明Lyso－PC可以选择性地诱导内皮细胞VCAM－1和ICAM-1的表达，提示Lyso－PC不仅能够趋化单核细胞，而且还能介导单核细胞与内皮细胞粘附，在动脉粥样硬化中起到重要作用。

Extensive monocyte recruitment is an early phenomenon associated with the development of atherosclerotic lesion, in which adhesion molecules expressed by endothelial cell play an active role. ln this study we examined the effects of Lysophosphatidylcholine (Lyso-PC), a major phospholipid component of atherogenic lipoproteins, on the expression of adhesion mo1ecules for monocytes, such as intercellular adhesion molecu1e-1 (Ⅰ-CAM-1),endothelial-leukocyte adhesion molecule-1 (E-selectin) and vascular cell adhesion molecule-1 (VCAM-1),in cultured human umbilical vein endothelial cells (HUVECs). After treating HUVECs with different concentration Lyso-PC at different times, the expression of adhesipn molecules on the surface of HUVECs induced by Lyso-PCwere analyzed using flow cytometry. The results showed that compared with control group, the surface expression of ICAM-1 and VCAM-1 was increased on HUVECs treated with Lyso-PC. ICAM-1 and VCAM-1 expression was maximally induced 1 h after stimulated with 50 pg/mg Lyso-PC, compared to basal values. At the 3 h after stimulation, ICAM-1 dropped to basal level while VCAM-1 dropped below to basal level. The effects of Lyso-PC on Eselectin expression were negligible with in 3 h. However, at the 3 h of treatment with 50μg/ml, the E-selectin expression on HUVECs began to be up-regulated significantly. We conclude that in addition to its monocyte chemoattractant activity, Lyso-PC can selectively induce ICAM-1 and VCAM-1 expression on the surface of HUVECs.Lyso-PC may play an important role in monocyte recruitment into athersclerotic lesions.