人工生物胰治疗糖尿病的实验研究

An Investigation of Bioartificial Pancreas for Treatment of Diabetes Mellitus

为防止胰岛移植后的排斥反应，本实验以大鼠胰岛、琼脂糖和胶原为原料制成人工生物膜（Bioartitcialpancreas，BAP），并在体内外条件下，研究其胰岛素的分泌功能、免疫保护性以及在异种动物糖尿病治疗中的作用。结果表明，此人工生物胰能维持胰岛的分泌功能、降低胰岛移植物的免疫原性、移植后能纠正糖尿病小鼠的糖代谢紊乱、维持正常血糖水平。提示以胰岛、琼脂糖和胶原制成的人工生物胰不失为治疗糖尿病，减缓异种胰岛移植排斥反应的一种有效手段。

Bioartificial pancreas (BAP) made up of rat islet, agarose and collagen was developed in this experiment to cure the diabetes mellitus and prevent immune rejection of the islet xenograft. The insulin secretion function, immunoprotection and effect of BAP on the treatment of diabetes mellitus in mice were investigated. The results showed that the BAP maintained the insulin secretion function of islet, decreased the immunogenicity of islet xenograft and normalized the metabolic disturbances of carbohydrate in diabetic mice. These indicate that the BAP composed of the islet, agarose and collagen is an alternate approach to therapy for diabetes mellitus and to remission of immune rejecction of the isIet xenograft.