发酵菜籽粕高产纳豆激酶菌株的选育研究

Breeding of A Producing High Vitality Bacillus natto Kinase Strains with Fermenting Rapeseed Meal

[目的]为了选育发酵菜籽粕高产纳豆激酶的优良菌株。[方法]出发菌株纳豆芽孢杆菌(Bacillus natto)Z1经过紫外诱变处理后,以菜籽粕作为唯一氮源进行摇瓶发酵培养,并以纳豆激酶为考核指标进行高产菌株的筛选。[结果]通过反复筛选和遗传稳定性试验,获得高产纳豆激酶且遗传稳定的突变株U49,其酶活力比Z1提高了100.34%。[结论]该研究可为利用菜籽粕工业发酵生产纳豆激酶奠定基础,并为新型保健食品和药物的产业化提供理论依据和技术支持。

[ Objective ] In order to breeding a producing high vitality Bacillus natto kinase strains with fermenting rapeseed meal. [ Method] The original strain Bacillus natto Z1 was accepted which suffered ultraviolet radiation mutation for decompressing bottle fermentation cultivation with rapeseed meal as the only source of nitrogen and then mutant strains was screened to get a strain which could produce high vitality Bacillus natto kinase. [ Result] By repeatedly screening and genetic stability testing, target strain named U49 was gained which enzyme activity im- proved above 100.34% in comparison to original strain Z1. [ Conclusion] The study lays a basis for utilizing rapeseed meal to produce Bacillus natto kinase by industrial fermentation, and provides a theoretical and technical support for industrialization of new types of health food and