碱性成纤维细胞生长因子对兔急性高眼压视神经纤维损害的保护作用

The ability of bFGF to protect rabbit optic nerve fibers in acute ocular hypertension

目的 观察急性高眼压对视神经的损害和碱性成纤维细胞生长因子 (basic fibroblast growthfactor,b FGF)对视神经的保护作用。 方法 计算机图像分析技术定量分析视神经纤维数量。经前房灌注平衡液的方法 ,制造兔急性高眼压模型。应用 b FGF玻璃体内注射后 ,观察视神经纤维数量变化。 结果 视网膜缺血组视神经纤维数量较正常明显减少 (P=0 .0 0 0 0 3) ,b FGF治疗组视神经纤维数量较高眼压组明显增多 (P=0 .0 0 78)。 结论 急性高眼压视网膜缺血能造成视神经纤维数量明显减少。玻璃体内注射b FGF能减轻急性高眼压对视神经纤维所造成的损害。

Objective [WT5”BZ]To observe the affection of optic nerve under acute ocular hypertension and the effect of protection of bFGF on optic nerve. [WT5”HZ]Methods [WT5”BZ]BSS was perfused into anterior chamber of rabbits to increase the intraocular pressure to cause retinal ischemia. A computer image analysis system was used to count the optic nerve axons.Eyes were intravitreally injected with bFGF and then the number of optic nerve axons of the normal rabbits,and hypertension with and without bFGE treatment groups were counted respectively. [WT5”HZ]Results [WT5”BZ]The number of optic nerve axons in ocular hypertension eyes was less than the normal eyes(P=0.000 03).The bFGF treated eyes had more optic nerve axons than the controls(P= 0.007 8). [WT5”HZ]Conclusions The acute ocular hypertension may cause the loss of the nerve axons,and bFGF may be effective in protecting optic nerve in acute ocular hypertension. [WT5”HZ]