摘要
实验大鼠预先分别给予不同药物后置于相同的热环境中，实施热损伤，同时观察大鼠体温升高速率。损伤后立即检测纹状体中 Ｄ_１Ｒ的平衡解离常数和最大结合容量，测定纹状体中ｃＡＭＰ的浓度，检测纹状体细胞中的钙调素（ＣＳＭ）的相对活性，从 Ｄ_１Ｒ及其信号转导的角度，探讨多巴胺受体影响体温调节的机制。结果表明，多巴胺受体激动剂左旋多巴能明显加快动物升温，而Ｄ_１Ｒ桔抗剂ＳＣＨ２３３９０则明显阻止升温；多巴胺两种受体分别具有相互桔抗的促进动物体温升高和阻滞体温升高的作用，神经活性物质神经节昔脂由于其稳定生物膜的作用，而在热损伤模型中同样具有损伤保护作用。
The effects of heat injury on D_1 receptor in rat striatum were observed, and the mechanism of the effect of dopamine receptor on thermoregulation was probed. Rats were put into hot environment after treated with L-DOPA, SCH23390, or ganglioside, and the rat body temperature increment was analyzed. At the same time, the Kd and Bmax of D_1 dopamine receptor in the rat striatum were identified with radioligand binding assay. The concentration of cAMP and the content of CaM were respectively examined by redio-competitive binding reaction and FACS. The results showed that L-DOPA accelerated the body temperature raise of rat suffering heat stress, and the antagonist SCH23390, however, blocked its increase. The stabilization effect of ganglioside on cell mem- brane could protect the striatum cell from heat injury as in other injury models.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2000年第3期174-176,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助课题!(编号39670627)
关键词
热损伤
D1受体
体温调节
大鼠
信号转导
heat injury
dopamine receptor thermoregulation
anatagonist
agonist
