摘要
研究腺病毒介导人野生型ｐ５３抑癌基因对肝癌细胞系ＨｅｐＧ２生物学行为的影响。构建含人野生型Ｐ５３抑癌基因的重组腺病毒载体，导入肝癌细胞系ＨｅｐＧ２后，分别用光镜、电镜、ＴＵＮＥＬ法检测细胞凋亡的发生和在裸鼠体内的成瘤性改变。肝癌细胞ＨｅｐＧ２导入野生型ｐ５３抑癌基因后，出现细胞凋亡，并对化疗药物顺铂的敏感性明显增加，且其生长受到一定程度的抑制，在裸鼠体内的成瘤性也降低。腺病毒介导人野生型ｐ５３抑癌基因能抑制肝癌ＨｅｐＧ２细胞的生长和在裸鼠体内的成瘤性，并诱导其发生凋亡，且对化疗药物顺铂的敏感性增加。
To investigate the change of biological behavior influenced by adenovirus-mediated transduction of human wild-type p53 gene in liver cancer cell line HepG2. By construction of recombinant adenovirus containing human p53 (named Ad-p53), apoptosis was detected using light microscopy and electron microscopy and TUNEL assay, and the tumorigenicity in nude mice was analysed in Ad-p53-transduced liver cancer cell lines. Ad-p53 could induce apoptosis, and elevate the sensitivity of human liver cancer cell line HepG2 to chemotherapy drugs cisplatin. The growth was inhibited and the tumorigenicity was reduced in Ad-p53-transduced liver cancer cell lines.Ad- p53 could inhibit the growth, reduce tumorigenicity in nude mice, and act synergetically with cisplatin in promo- tion of inducing apoptosis in liver cancer cell line HepG2.
出处
《解放军医学杂志》
CSCD
北大核心
2000年第3期185-188,共4页
Medical Journal of Chinese People's Liberation Army
