老年大鼠耳蜗核乙酰胆碱受体的表达

EXPRESSION OF ACETYLCHOLINE RECEPTOR IN AGED RAT COCHLEAR NUCLEUS

分别自青年和老年大鼠耳蜗核提取多聚腺嘌呤信使核糖核酸（ｍＲＮＡ），注入非洲爪蟾卵母细胞表达有功能的乙酸胆碱（ＡＣｈ）受体，利用电压钳方法记录ＡＣｈ受体电流。在注射老年大鼠耳蜗核ｍＲＮＡ的卵母细胞膜上记录到ＡＣｈ受体电流最大幅度＜青年组（Ｐ＜０．０５）。推论移植的老年大鼠耳蜗核ＡＣｈ受体功能的下降可能与老年性聋的发病有关。

Poly(A)~ + mRNAs isolated from the cochlear nucleus of the adolescent(2-3 months) and aged (24 -36 months) rats were microinjected into Xenopus laevis oocytes to express the functional acetylcholine receptor. Voltage clamp technique was used to record the current of acetylcholine receptor. The maximum amplitude of ace- tylcholine receptor current detected in aged group was lower than that in adolescent group (P<0. 05). The result indicates that the decrease of acetylcholine receptor function in aged rat cochlear nucleus may be related to presby- cusis.