腋窝淋巴结阴性乳腺癌淋巴结微小转移及c-erbB-2扩增的研究

Study on Micrometastases in Axillary Lymph nodes and c-erbB2 Amplification in Axillary Node-negative Breast Cancer

目的:探讨腋窝淋巴结阴性 (ANN)乳腺癌淋巴结微小转移 (LNM)以及原发灶c -erbB -2扩增的临床意义。方法:应用免疫组化方法对58例ANN乳腺癌LNM进行检测 ,利用差异PCR技术测定对应原发灶c-erbB -2扩增。结果:25.8 %病例存在LNM ,31.0 %伴有c-erbB -2扩增 ;在低分化乳腺癌组中 ,LNM阳性率明显高于高分化组 ;肿瘤直径>2.0cm者的LNM检出率显著高于直径≤2.0cm患者 ;在LNM阳性组中 ,c -erbB -2扩增率明显高于阴性组。结论:免疫组化可大大提高乳腺癌病人微小转移检出率 ,c -erbB -2扩增在乳腺癌转移早期即可发生。

Objective: To explore axillary lymph nodes metastases (LNM) and c-erbB2 amplification in axillary node-negative (ANN) breast cancer and its clinical significance. Methods: The LNM was examined in 58 cases of ANN breast cancer by immunohistochemistry. The c-erbB2 amplification of tumor tissues was studied in the same patients by differential PCR. Results: The positive rate of LNM and c-erbB2 amplification were 25.8% and 31.0% respectively. In the poorly differentiated group, the positive rate of LNM was significantly higher than that of well differentiated group. In the cases of tumor >2cm, LNM rate was obviously higher than that of < 2cm. In the LNM positive group, c-erbB2 amplification rate was significantly higher than that of negative group. Conclusion: The data suggest that immunohistochemical technique can greatly facilitate the detective rate of micrometastases, and c-erbB2 amplification usually occurs at the early stage of cancer metastases.