摘要
目的：研究分光光度法测定大豆磷脂中磷的含量．方法：将样品中的有机磷酸化合物分解转化为无机磷酸盐，再利用在酸性溶液中磷酸盐与铜酸接及Fiske－Subbarow试剂作用生成蓝色的化合物，在660um处用分光光度法测定．结果：磷的浓度在0．04ug／ml－0．16ug／ml之间线性关系良好，相关系数r＝0．9999，平均回收率为100．1％，RSD=0．57％（n=9）．结论：本方法简便、准确、重现性好．
Objective: To develop an assay for the determination of phosphorus level in soya lecithin by spectrophotometry Methods: Spectrophotometry was se lected to determine the phospho rus l e ve I in soya l e c ith in, based on the fo rmation of the blue complex by phosphate with ammonium molybdate and Fiske-Subbarow reagent in acid solution. Resulitsf The linearity for phosphorus was ranged over 0.04-0.16ug/ml with r=0.9999, the average recovery of 100. 1% and RSD of 0.57% (n=9). Conclusion The method was proved to be simple, precise and reproduciable.
出处
《黑龙江医药科学》
2000年第1期21-21,共1页
Heilongjiang Medicine and Pharmacy
关键词
分光光度法
磷
大豆磷脂
测定
soya lecithin, phosphorus, spectrophotometry
