CLU基因干扰肾癌786-O细胞差异基因表达的研究

Interference in the expression of differential genes of human renal cancer cell line 786-O by CLU gene

目的:观察CLU基因干扰前后肾癌细胞株786-O的基因表达谱差异,探讨肾细胞癌与CLU基因相关基因及传导通路。方法:CLU基因干扰慢病毒载体及空病毒载体转染肾癌786-O细胞样本与RocheNimbleGen表达谱芯片杂交,比较干扰前后基因表达谱差异,并行GO分析及Pathway分析。结果:六份样本中,共筛选出差异表达基因1731个,其中共同上调基因1154个,共同下调基因577个。差异表达基因中生物进程、细胞成分、分子功能方面均以下调为主。通路分析显示29条通路上调,如细胞黏附、异体排斥、吞噬体。31条通路下调,如mTOR、MAPK、非小细胞肺癌。结论:采用全基因组芯片检测CLU基因干扰后肾癌786-O基因表达谱获得差异表达基因,为以CLU基因为靶点的肾癌基因研究提供了实验依据。

Objective To investigate the gene expression profiles of human renal cancer cell line 786-0 before or after lentivirus-mediated clusterin silence by gene expression array. Methods 3 samples treated with CLU-RNAi-LV and 3 samples with negative control LV were labeled with Cy3 fluorescence as probes. The mixed probes were hybridized with gene chip. Differentially expressed genes screening were detected by mieroarray scanner. Results There were 1 731 differentially expressed genes in the 6 samples, among which 1 154 were up- regulation genes and 577 were down-regulation genes. GO analysis showed that the numbers of down-regulated genes in biological processes, cellular component and molecular function were significantly higher than those of up- regulated genes. The top 3 up-regulation enrichment pathways were cell adhesion molecules, allograft rejection and phagosome, whereas the top 3 down-regulation pathways were roTOR signaling pathway, MARK signaling and non- small cell lung cancer. Conclusion Differentially expressed genes screening before or after clusterin silence in 786-0 showed that CLU gene plays an important role in the renal cancer＇s occurrence and development.