TNF与化疗药物不同配伍对胃癌细胞作用的研究

Experimental Studies of Correct Regimen of Tumor Necrosis Factor Combined with Antitumor Drugs on Gastric Cancer Cell

目的 研究肿瘤坏死因子 (TNF)、5 氟脲嘧啶 ( 5 Fu)、丝裂霉素 (MMC )单用及TNF与 5 Fu、MMC不同配伍方案对人胃癌细胞SGC 790 1的细胞毒作用。方法 应用噻唑蓝比色法 (MTT )测量各实验组的光密度值 (OD值 ) ,计算杀伤率。结果 单独应用各药时 ,三者对SGC 790 1均有细胞毒作用 ,TNF与 5 Fu、MMC配伍时细胞毒作用明显增强 (P <0 .0 1) ,不同配伍方案存在非常显著的差异 (P <0 .0 1) ,以先给化学药物、后加TNF组毒性作用最强。结论 TNF能成倍提高化学药物的细胞毒作用 ,且应在先使用化学药物的基础上给药。

Objective To study the cytotoxicity of different regimen of tumor necrosis factor(TNF) combined with antitumor drugs on human gastric cancer cell line SGC 7901.Methods To measure optical density value(OD) by MTT assay,calculate killing rate.Results TNF increased the cytotoxicity of antitumor drugs.Among three regimens,there was significant difference in cytotoxicity( P <0.01).In three combined administrations,the most effective one was that TNF was given 12 h later post treatment of antitumor drugs.Conclusion TNF can remarkably increase the cytotoxicity of antitumor drugs.In clinical therapy,we should give TNF after antitumor drugs administration.