一氧化氮信息传递系统对大鼠呼吸道纤毛运动的影响

Effect of NO signal system on rat airway ciliary motility

目的 了解一氧化氮 (NO)信息传递系统对呼吸道上皮细胞纤毛运动频率 (CBF)的影响。方法 选用雄性SD大鼠 9只 ,异氟醚麻醉后以无菌技术迅速取其气管 ,并将气管粘膜切成1mm2大小的组织块 ,在DMEM中进行组织培养。将组织块分成 5组 :L 精氨酸 (L Arg)组 ;1 Hy droxy 2 oxo 3 (N ethyl 2 aminoethyl) 3 ethyl 1 triazene (NOC 12 ,NO载体 )组 ;8 Br cGMP组 ;D Arg组及磷酸盐缓冲液 (PBS)组。以相差显微镜及成像分析技术测定CBF ,对纤毛运动进行定量分析。结果 (1)给L Arg后CBF由 (7 43+0 75 )Hz增至 (8 5 9+0 93)Hz(P <0 0 1) ;给NOC 12后CBF由 (7 6 6 +0 6 8)Hz增至 (8 6 2 +0 91)Hz(P <0 0 5 ) ;给 8 Br cGMP后CBF由 (7 11+0 6 6 )Hz增至(8 10 +0 83)Hz(P <0 0 5 ) ;(2 )PBS及D Arg对CBF无影响。 结论 NO信息传递系统能使大鼠呼吸道上皮细胞CBF加快。

Objective To understand the effects of NO signal system on the ciliary beating frequency (CBF) of airway epithelial cellMethods Nine normal male Sprague-Dawley rats were anesthetized with isoflurane Their tracheas were rapidly removed using aseptic technique The mucosa of trachea were cut into 1mm2 explants and cultured in DMEM The explants were divided into 5 groups as bellow: L-Arg group, 1-Hydroxy-2-oxo-3-(N-ethyl-2-aminoethyl)-3-ethyl-1-triazene (NOC-12) group, D-Arg group, 8-Br-cGMP group, and phosphate buffered saline (PBS)group Actively beating ciliated cells were observed, and their motion was quantified by measuring CBF using phase-contrast microscopy and videotape analysis Results L-Arg increased CBF from (7 43±0 75)Hz to(8 59±0 93)Hz (P<0 01),NOC-12 increased CBF from (766±068) Hz to (862±0 91)Hz (P<005), and 8-Br-cGMP increased CBF from (711±066)Hz to (810±0 83)Hz (P<005)PBS and D-Arg did not change CBF significantlyConclusions NO signal pathway increases the CBF of airway epithelial cell