摘要
ObjectiveSeveral 研究显示了 miR-15a， miR-15b 和 miR-16 可以是 apoptosis 的重要管理者。后来稀释 apoptosis 能保护心肌层和还原剂梗塞尺寸，现在的学习被瞄准发现是否这些 miRNAs 参予调整心肌的局部缺血灌注( I/R )在受到 I/R 的老鼠心的 injury.MethodsApoptosis 被 TUNEL 试金在 vivo 检测，当流动 cytometry 分析由 Annexin V/PI 列在后面时，在 vitro 的两倍污点被用来在受到 hypoxia/reoxygenation ( H/R )的有教养的 cardiomyocytes 检测 apoptosis 。Taqman 即时量的 PCR 被用来证实 miR-15a/15b/16 是否涉及心脏的 I/R 的规定，控制， I/R 或 H/R 的到那些的 H/R.ResultsCompared 导致了 cardiomyocytes 的 apoptosis 显著地在 vivo 两个都被增加(24.4%±；9.4% 对 2.2%±；1.9% ， P <；0.01， n = 5 ) 并且在 vitro (14.12%±；0.92% 对 2.22%±；0.08%) 。 miR-15a 和 miR-15b 的表达式，然而并非 miR-16 ，在鼠标 I/R 模型，和结果被增加在 H/R model.ConclusionsOur 数据是一致的显示因此， miR-15 和 miR-15b 响应心脏的 I/R 损害是起来调整的 miR-15a/b 的下面规定可以是有希望的策略减少心脏的 I/R 损害导致的心肌的 apoptosis 。
Objective Several studies have indicated that miR-15a,miR-15b and miR-16 may be the important regulators of apoptosis.Since attenuate apoptosis could protect myocardium and reduce infarction size,the present study was aimed to find out whether these miRNAs participate in regulating myocardial ischemia reperfusion （I/R） injury.Methods Apoptosis in mice hearts subjected to I/R was detected by TUNEL assay in vivo,while flow cytometry analysis followed by Annexin V/PI double stain in vitro was used to detect apoptosis in cultured cardiomyocytes which were subjected to hypoxia/reoxygenation （H/R）.Taqman real-time quantitative PCR was used to confirm whether miR-15a/15b/16 were involved in the regulation of cardiac I/R and H/R.Results Compared to those of the controls,I/R or H/R induced apoptosis of cardiomyocytes was significantly iucreased both in vivo （24.4％ ± 9.4％ vs.2.2％ ± 1.9％,P ＜ 0.01,n =5） and in vitro （14.12％ ±0.92％ vs.2.22％ ± 0.08％）.The expression of miR-15a and miR-15b,but not miR-16,was increased in the mice I/R model,and the results were consistent in the H/R model.Conclusions Our data indicate miR-15 and miR-15b are up-regulated in response to cardiac I/R injury,therefore,down-regulation of miR- 15a/b may be a promising strategy to reduce myocardial apoptosis induced by cardiac I/R injury.
