蝉蜕不同提取工艺抗凝与纤溶活性比较研究

Comparison of Anticoagulant and Fibrinolytic Activities of Cicada Slough by Different Extraction Methods

目的:比较蝉蜕不同提取工艺抗凝与纤溶活性。方法:以凝血酶原时间试验(PT)、活化部分凝血活酶时间试验(APTT)、血小板聚集率和血凝-纤溶动态图为指标,比较蝉蜕仿生胃/胰酶解物、水提物、醇提物、水提醇沉物的抗凝与纤溶活性差异,综合评价各提取物抗凝纤溶活性。结果:蝉蜕各提取物均不能延长家兔PT值,而仿生胃/胰酶解物和水提醇沉(渣)对于APTT、血小板聚集率、血凝-纤溶表现出抗凝纤溶作用。结论:蝉蜕仿生胃/胰酶解物和水提醇沉(渣)具有一定抗凝纤溶活性,具有后续研究价值。

Objective： To compare the anticoagulant and fibrinolytic activities of cicada slough by different extraction methods.Methods：It was taken the prothrombin time（PT）,activated partial thromboplastin time（APTT）,platelet aggregation rate,and blood clots-fibrinolytic dynamic figure as the indexes,compare and evaluate the anticoagulant and fibrinolytic activities of cicada slough by four different extraction methods,which were bionic pepsin/trypsin enzymatic hydrolysis,alcohol refluxing,boiling water extract and water extract with alcohol precipitate,comprehensively.Result： All the extracts couldn＇t obviously prolong PT.On the results of APTT,platelet aggregation rate,and blood clots-fibrinolytic dynamic figure,the extracts of bionic pepsin/trypsin enzymatic hydrolysis and the sediment of water extract with alcohol precipitate were demonstrated a relatively good anticoagulant and fibrinolytic activities.Conclusion： The cicada slough extracts of bionic pepsin/trypsin enzymatic hydrolysis and the sediment of water extract with alcohol precipitate are valuable to be the follow-up study objects for anticoagulant and fibrinolytic activities field.