摘要
目的:研究调督通脉针灸法对佐剂性关节炎(AA)大鼠膝关节滑膜中TNF-α、IL-10表达的影响,以探讨类风湿关节炎(RA)发病机制,为针灸治疗RA提供理论依据。方法:完全弗氏佐剂诱导Wistar大鼠AA,观察AA大鼠多发性关节炎指数的变化,采用反转录-聚合酶链反应(RT-PCR)法检测膝关节滑膜中TNF-α、IL-10的表达水平。结果:针灸第7天和第14天,针灸能明显抑制大鼠多发性关节炎指数(P<0.05,P<0.01),且调督通脉针灸法优于普通针灸法(P<0.05);针灸第14天采集标本,RT-PCR法观察到针灸能降低AA大鼠膝关节滑膜中TNF-α的表达水平,提升IL-10的表达水平,且调督通脉针灸法优于普通针灸法(P<0.05)。结论:调督通脉针灸法能够降低AA大鼠膝关节滑膜中TNF-α的表达,提升IL-10的表达水平,具有双向调节作用,为针灸临床治疗RA提供理论依据。
Objective： To explore the expressions of Tiaodu Tongmai acupuncture for TNF-α and IL-l0 in the Adjuvant induced arthritis（AA） rats synovial of knee joint,to discuss the nosogenesis of rheumatoid arthritis（RA） and provide theoretical foundation for RA acupunture therapy.Methods： Complete Freund＇s adjuvant inductive Wistar AA rats,and observe multiple arthritis index score.Observe the expression of TNF-αand IL-l0 in synovial of knee joint by anti-retroviral-polymerase chain reaction（RT-PCR）.Results： On the 7th and 14th day during the acupuncture course,acupuncture could reduce multiple arthritis index score of AA rats（P0.05,P0.01）,and the Tiaodu Tongmai acupuncture was better than the conventional acupuncture（P0.05）.Conclusion： The Tiaodu Tongmai acupuncture therapy can reduce the expression of TNF-α and can increase the expression of IL-l0,which means two-ways regulation function,and it provides the theoretical foundations for clinical treatment for RA acupuncture.
出处
《中医药信息》
2012年第6期88-90,共3页
Information on Traditional Chinese Medicine
基金
黑龙江省中医药管理局科研项目(ZHY10-W17)
