花生中黄曲霉毒素检测方法的研究

The determination of aflatoxins in peanuts

目的:通过采用免疫亲和柱-高效液相色谱法来测定花生中的黄曲霉毒素。方法:样品用甲醇+水(70∶30)提取液提取后过滤,滤液经黄曲霉毒素免疫亲和柱净化,洗脱液经N2吹干后,加三氟乙酸和正己烷衍生后,用带有荧光检测器的超高效液相色谱仪测定。结果:四种黄曲霉毒素G2、G1、B2、B1得到很好的分离。检测限分别为:0.2μg/kg、0.4μg/kg、0.2μg/kg、0.4μg/kg。平均回收率在:81.5%～106.2%,相对标准偏差在1.1%～3.5%。结论:该法能使花生中黄曲霉毒素G2、G1、B2、B1得到很好的分离,结果准确。

Objective：To determine aflatoxins in peanuts by using high performance liquid chromatography-fluorescence detector and immuno-affinity column.Methods： The sample was extracted with V（H2O）∶ V（CH3OH）=30∶ 70,then separated by a immuno-affinity column after filteration.The purified solution was concentrated and evaporated to dryness.Aflatoxins were derivatized with TFA and detected and quantitated by HPLC with fluorescence detector.Results： Four aflatoxins G2,G1,B2,B1 were well separated.Detection limits were： 0.2 μg/kg,0.4 μg/kg,0.2 μg/kg,0.4 μg/kg.The average recovery rate： 81.5%~106.2%.The relative standarddeviation：1.1%~3.5%.Conclusion： This method can in make aflatoxins G2,G1,B2,B1 well separated in peanuts in a short time with accurate test result.