多药耐药鲍曼不动杆菌主动外排作用研究

Role of efflux system on multi-resistance of acinetobacter baumannii

目的:观察羰基氰氯苯腙(CCCP)对多药耐药鲍曼不动杆菌耐药水平影响,以探讨主动外排作用在鲍曼不动杆菌耐药中所起的作用。方法:用琼脂二倍稀释法测定鲍曼不动杆菌对哌拉西林等临床常用抗生素的最低抑菌浓度(MIC),观察在CCCP存在条件下MIC值的变化;用PCR法扩增外排泵编码基因adeB。结果:79.2%(19/24)的菌株外排泵表型试验阳性,其中有63.2%(12/19)同时对2种以上的药物有明显的外排作用,这19株主动外排表型阳性菌株adeB基因检测均阳性。结论:外排泵抑制剂在体外能降低多种抗菌药物对鲍曼不动杆菌的MIC,外排泵抑制剂的应用及外排泵编码基因的检测为临床提供一种敏感的检测鲍曼不动杆菌主动外排系统的方法。

Objective：To investigate the effect of carbonyl cyanide 3-chlorophenyl-hydrazone（CCCP） on the multiple resistance of Acinetobacter baumannii,the encoding gene for efflux pump and the relationship between efflux pumps and resistance of Acinetobacter baumannii.Methods： Two fold agar dilution method was used to determine the minimum inhibitory concentrations（MIC） of 8 kinds of antibiotics such as Piperacillin of acinetobacter baumannii strains,and the variation of MIC was observed with CCCP.PCR was used to detect the adeB genes.Results： 79.2%（19/24） of the strains were positive in phenotype tests of efflux pump.63.2%（12/19） of the strains were found to have an evident exfflux effect against more than two antibiotics.AdeB gene could be found in the 19 strains of Acinetobacter baumannii.Conclusion： Efflux pump inhibitors may reduce the MIC of some antibiotics in vitro.In certain cases,the efflux inhibitor could be used to overcome the bacterial resistances in clinic.