地西他滨联合丙戊酸钠对AML细胞株U937促凋亡和分化的影响

Synergistic effect of decitabine and valproic acid on induction of apoptosis and differentiation in leukemic U937 cells

目的:探讨地西他滨(decitabine,DCA)和丙戊酸钠(valproic acid,VPA)联用对白血病细胞株U937的促凋亡和分化影响。方法:设立分组如下:对照组,DCA单药A组(1.0μmol/L),DCA单药B组(4.0μmol/L),VPA单药组(2.0 mmol/L),联合用药A组(DCA 1.0μmol/L+VPA 2.0 mmol/L),联合用药B组(DCA 4.0μmol/L+VPA 2.0 mmol/L),作用48 h。应用Annexin V-FITC/PI标记法检测早期凋亡率,流式细胞术检测CD34、CD117 MFI以及CD11b、CD14表达率。结果:联合用药组A、B的凋亡率高于其各自的单药组,差异具有显著统计学意义(P<0.01);联合用药A、B组的CD117和CD34 MFI低于其各自的单药组,差异具有显著统计学意义(P<0.01);联合用药A、B组的CD11b和CD14表达率低于其各自的单药组,差异具有显著统计学意义(P<0.01)。结论:U937细胞中VPA能显著增强DCA的促凋亡分化作用。

Objective：To investigate the synergistic effect of decitabine（DCA） and valproic acid（VPA） in apoptosis and differentiation induction in leukemic U937 cells.Methods： The drug groups were set as follows：control group;DCA alone group A（1.0 μmol/L）;DCA alone group B（4.0 μmol/L）;VPA alone group（2.0 mmol/L）;combination group A（DCA 1.0 μmol/L＋VPA 2.0 mmol/L）;combination group B（DCA 4.0 μmol/L＋VPA 2.0 mmol/L）.The cells were treated by drug for 48 hours.The early apoptosis rates were detected by staining with Annexin V and PI.The CD117,CD34 mean fluorescence intensity（MFI） and CD14,CD11b expression were detected by flow cytometry.Results： The apoptosis rates of combination group A and B were significantly higher than their corresponding concentration single drug group（P0.01）.The CD117,CD34 MFI of combination group A and B were significantly lower than their corresponding concentration single drug group（P0.01）.The CD14,CD11b expressions of combination group A and B were significantly lower than their corresponding concentration single drug group（P0.01）.Conclusion： There was an enhanced antileukemia activity of combinations of DCA and VPA on apoptosis and differentiation of U937 cells.