醋酸纤维素薄膜电泳法分离兔血清蛋白质

The separation of rabbit serum proteins by cellulose acetate membrane electrophoresis

目的:采用醋酸纤维素薄膜电泳法确定分离兔血清蛋白质的最佳实验条件,并测定蛋白各组分的含量。方法:对醋酸纤维素薄膜电泳法的电压和时间进行筛选,随机组合确定实验条件。结果:应用醋酸纤维膜电泳法可将兔血清蛋白质分为五条区带,其中,清蛋白含量最高,α1球蛋白>β球蛋白>α2球蛋白,而γ球蛋白的含量高于α和β球蛋白。结论:应用醋酸纤维膜电泳法分离兔血清蛋白质,在电压120 V或150 V、时间50 min(夏季40 min)条件下进行。该方法对蛋白分离效果好、结果稳定,适用于实验教学。

Objective：To determine the best experimental conditions for separation of rabbit serum proteins,and detect each protein content using cellulose acetate electrophoresis method.Methods： The voltage and time on cellulose acetate electrophoresis were screened by rational combination to find the best experimental conditions.Results： The rabbit serum proteins can be divided into five district zones,in which the highest content of proteins was albumin,followed by α1 globulin,β and α2 globulin.Besides,the content of γ-globulin was higher than the α and β globulin by the cellulose acetate membrane electrophoresis.Conclusion： An effective method for separation of rabbit serum proteins was established,and the condition was that the voltage was 120 V or 150 V,and the time was 50 min（40 min in summer）,which can provide the good protein separation and stable results,it is suitable for experimental teaching.