贵阳地区2010年手足口病病原体检测分析

Analysis of pathogens of the hand foot and mouth diseases in Guiyang in 2010

目的:对贵阳市2010年手足口病流行期间的手足口病患儿进行病原学及流行特征分析。方法:采用Real-TimePCR法检测307例手足口病患儿标本样本中的肠道病毒通用型(EV)、肠道病毒71型(EV71)、肠道病毒科萨奇A16型(CoxA16)3种核酸,将扩增产物测序获得的序列与GenBank中肠道病毒核苷酸序列进行比对,确定毒株血清型别。结果:肠道病毒通用型(EV)阳性率为68.27%,CA16阳性率为6.73%,EV71阳性率为13.46%。重症患者EV71的检出率为18.60%,CoxA16的检出率为4.65%,其他肠道病毒为57.55%,4株其它肠道病毒经鉴定属于CA10型。结论:手足口病病原复杂,2010年贵阳地区HFMD的主要病原为其他肠道病毒和EV71,存在一定比例的其他未知病原体(CoxA10)。

Objective：To study the genotype of enterovirus and the epidemiological characteristics of hand,foot and mouth disease（HFMD） among children in Guiyang during the HFMD epidemic period in 2010.Methods： Nucleic acid of enterovirus popular type,enterovirus 71（EV71）and coxsckievirus A16（CoxA16） were detected by Real-Time PCR methods in 307 HFMD samples.PCR products were sequenced and aligned to Genbank to determine the serotypes of the viruses.Results： Positive rates of enterovirus,Coxsackie A16 and Enterovirus 71 were 68.27%,6.73% and 13.46%,respectively.In all severe HFMD cases,the detection rate of EV71 was 18.60%,the detection rate of CoxA16 was 4.65%,other Enterovirus was 57.55%.4 untyped EV were identified as CA10 respectively.Conclusion： The causative agents of HFMD are complicated.The results showed that other enterovirus and EV71 were the major pathogens for the epidemics of HFMD in 2010 in Guiyang,while the pathogens in some patients are still unknown,such as CoxA10.