猪STAT4、STAT6基因克隆及组织表达研究

Signal transducer and activator of transcription 4 and 6 genes in pigs(Sus scrofa):cDNA cloning and tissue expression

本研究以长白猪为材料,克隆了STAT4和STAT6基因的cDNA全长,其中STAT4基因cDNA全长2269 bp,编码748个氨基酸的前体蛋白,与人、牛、大鼠、小鼠等哺乳动物STAT4氨基酸序列一致性分别为97%、98%、96%、96%;STAT6基因cDNA全长2637 bp,编码847个氨基酸的前体蛋白,与人、牛、大鼠、小鼠等哺乳动物的氨基酸序列有很高的同源性,分别为93%、95%、88%和87%.采用RT-PCR方法,本研究对家猪STAT4和STAT6基因进行组织表达分析.结果显示:STAT4在所有组织中都有表达,STAT6在心、肝、脾、肺、肾、肌肉、小肠等组织中有表达.此外,本研究已将家猪STAT4和STAT6基因编码区序列克隆到真核表达栽体pcDNA3.1(+)中,并做了初步功能鉴定.

The full-length cDNAs of STAT4 and STAT6 have been cloned from adult pig liver. STAT4 cDNA encodes a protein precursor of 748 amino acids, which shares high amino acid sequence identity with that of human （97%）, cattle （98%）, rat （96%）, mouse （96%）. The full-length cDNA of STAT6 encodes a protein precursor of 847 amino acids which shares high amino acid sequence identity with that of human （93%）, cattle （95%）, rat （88%） and mouse （87%）. Using RT-PCR assay, the mRNA ex- pression of STAT4 and STAT6 genes were also examined in adult pig tissues. The results showed that they were widely expressed in nearly all of the tissues examined. In addition, the expression plasmids of pig STAT4 and STAT6 were also constructed in the present study. These data pave the way for further functional characterization of STAT4 and STAT6 genes in pigs.