miR-184及miR-205在绵羊不同生长时期毛囊及其他组织中的表达研究

The Study on Expression of miR-184 and miR-205 at Different Developmental Stages of Hair Follicle and Other Tissues of Sheep

为了研究miR-184及miR-205在绵羊毛囊发育过程中的作用,本研究以绵羊为研究材料,检测了miR-184及miR-205在毛囊3个发育时期及不同组织中的表达情况,进行了基因组定位及前体预测,并预测了可能的靶基因及调控的基因通路。结果发现,miR-184的表达量从毛囊生长期到衰退期呈逐步上升的趋势,而miR-205则表现为先上升后下降,其在衰退期表达量最高。2个microRNA在绵羊的多个组织中均有表达。两者的候选靶基因涉及15个基因通路,可能通过Wnt通路调控毛囊的生长周期。

In order to study the roles of miR-184 and miR205 in hair follicle development of sheep, the expression of miR-184 and miR-205 at three different developmental stages of hair follicle of sheep was detected by QPCR. The precursors of the two microRNAs was predicted and the sequences were mapped to genome of sheep. Furthermore target genes and pathways of the two microRNAs were predicted. The results indicated that the expression of miR-184 in hair follicle tissue showed increasing trend from anagen to telogen. However, the miR-205 was upregulated from anagen to catagen, and then downregulated from catagen to telogen. The expression of miR- 205 was highest in catagen, miR-184 and miR-205 both express in many different tiusses. Moreo- ver, the candidate target genes of the two microRNAs are involved in 15 different pathways and miR-184 and miR-205 probably regulate the hair follicle development through Wnt signaling path- way.