汉黄芩素对多发性骨髓瘤细胞周期及P-Wee1和P-Akt蛋白表达的影响

Wogonin Downregulates P-Wee1 and P-Akt Expression in RPMI8226 Cells to Inhibit Cell Proliferation

目的探讨汉黄芩素对多发性骨髓瘤RPMI8226细胞的增殖抑制作用及其可能的机制。方法以不同浓度的汉黄芩素(5～100μg/ml)作用于RPMI8226细胞24h,使用CCK-8法、流式细胞仪检测细胞的增殖活性和细胞周期的变化;运用Western-blot法检测汉黄芩素作用前后细胞内P-Wee1、P-Akt蛋白表达的变化。结果汉黄芩素对RPMI8226细胞具有增殖抑制作用,并有剂量依赖性,IC50为43.71μg/ml;汉黄芩素浓度为50μg/ml时,用药组的G2/M%为(25.4333±2.35433)%,明显高于溶剂对照组的(6.8333±1.52753)%;Western-blot检测结果显示,用药组汉黄芩素浓度≥50μg/ml时,与对照组相比,P-Wee1、P-Akt蛋白表达明显减少。结论汉黄芩素能明显抑制RPMI8226细胞的增殖,可能是通过Akt/Wee1途径使肿瘤细胞发生G2/M期阻滞,从而发挥增殖抑制作用。

Objective To investigate the effects of Wogonin on multiple myeloma cell line RPMI8226 and its possible mechanism. Methods CCK-8 assay was used to evaluate the proliferative activity of RPMI8226 treated with Wogonin （5-100 μg/ml） for 24 hours. Flow cytometry was used for the detection of cell cycle. P-Weel and P-Akt expression were detected by Western-blot. Results Wogonin could significantly inhibit the proliferation of RPMI8226 cells in a dose- dependent manner,IC50=43.71 μg/ml. Exposure to Wogonin （50 μg/ml） for 24 h induced a significant proportion of cells accumulated at the G2/M phase. P-Weel and P-Akt were decreased in Western-blot assay when Wogonin concentration was equal or greater than 50 μg/ml. Conclusions Wogonin suppressed the proliferation of RPMI8226 cells. Wogonin-induced cell cycle arrest at the G2/M phase might be mediated through Akt/Weel pathway.