虫草多糖的分离纯化及含量测定

Purification and determination of Cordyceps polysaccharide

目的采用发酵法从冬虫夏草发酵液及菌丝体中提取、分离虫草多糖。方法采用乙醇沉淀法分离冬虫夏草发酵液和菌丝体中的多糖;采用苯酚硫酸法测定样品中多糖含量。结果采用此法进行制备、检测,方法简单快速,结果准确,适宜推广使用。结论此法无需多糖纯品,具有快速简单、精确度高、灵敏度高、重现性较好的优点,是检测虫草多糖含量的较优方法之一。

Objective Using the method of inoculated fermentation to extract and separate the Cordyceps polysaccharide from the zymotic fluid and mycelium of Chinese caterpillar fungus. Methods Separated the polysaccharide of Chinese caterpillar fungus＇ zymotic fluid and mycelium in ethanol precipitation;survey and evaluate the polysaccharide content of samples in phenol-sulfuric acid method. Results It is profitable for using widely because of the fast, easy method and exact result for producing and detecting. Conclusion This method is one of excellent ways for detecting the Cordyceps polysaccharide contents and it doesn＇t need the polysaccharide from pure product. The advantages are fast and easy, high accuracy and sensitivity,and good reproducibility.