细胞因子及热休克蛋白在慢性前列腺炎患者精浆中的含量及其临床意义

Levels of cytokines and heat-shock protein 70 in the seminal plasma of patients with chronic bacterial prostatitis and chronic prostatitis/chronic pelvic pain syndrome

目的:探讨慢性细菌性前列腺炎以及慢性前列腺炎/慢性骨盆疼痛综合症(CP/CPPS)患者精浆中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)以及热休克蛋白70(HSP70)的含量及临床意义。方法:采用酶联免疫吸附测定法(ELISA)对36例慢性细菌性前列腺炎(Ⅱ型)、43例炎症型慢性非细菌性前列腺炎即炎症型慢性骨盆疼痛综合征(ⅢA型)、46例非炎症型慢性非细菌性前列腺炎即非炎症型慢性骨盆疼痛综合征(ⅢB型)患者及25例健康志愿者精浆中TNF-α、IL-1β以及HSP70含量进行测定分析,并将HSP70的表达与慢性前列腺炎症状评分进行相关性分析。结果:慢性细菌性前列腺炎患者精浆中细胞因子(TNF-α、IL-1β)以及HSP70含量明显高于CP/CPPS患者及正常对照组。CP/CPPSⅢA型患者精浆中IL-1β的含量明显高于CP/CPPSⅢB型患者及正常对照组。CP/CPPS患者精浆中HSP70的含量明显低于正常对照组。慢性细菌性前列腺炎患者精浆中HSP70的含量与CPSI评分呈显著负相关。结论:精浆中HSP70的含量能够作为评价慢性细菌性前列腺炎病情程度的分子生物学指标。IL-1β能够作为CP/CPPS分型诊断指标。对于慢性细菌性前列腺炎患者,HSP70具有细胞保护功能。CP/CPPS患者精浆中HSP70表达的抑制可能与T细胞功能受损有关。

Objective： To assess the diagnostic value and potentially protective capacity of tumor necrosis factor-α （TNF-α）, interleukin 1 β （IL-1 β） and heat-shock protein 70 （HSP70） in chronic bacterial prostatitis （CBP） and chronic prostatitis/chronic pel- vic pain syndrome （CP/CPPS）. Methods： We determined the levels of cytokines TNF-α, IL-1 β and HSP70 by ELISA in the semi- nal plasma of 150 men： 36 with CBP, 43 with CP/CPPS ⅢA, 46 with CP/CPPS ms, and 25 healthy controls. We analyzed the cor- relation of the HSP70 expression in the CBP and CP/CPPS patients with the chronic prostatitis symptom index （CPSI）. Results ： Sig- nificantly increased levels of TNF-α, IL-1β and HSP70 were observed in the seminal plasma of the CBP patients as compared with theCP/CPPS patients and healthy controls. The expression of IL-1β was significantly higher in the patients with CP/CPPS Ⅲ A than in those with CP/CPPS ⅢB and the controls, while the HSP70 level remarkably lower in those with CP/CPPS than in the controls, and its concentration in the seminal plasma of the CBP patients was negatively correlated with CPSI. Conclusion ： The levels of HSP70 and IL-1β in the seminal plasma appear to be most reliable molecular biological markers for the diagnosis of CBP and CP/CPPS, respective- ly. HSP70 has an important protective role in the regulation of cell functions in CBP patients. CP/CPPS is probably detrimental to the function of T cells and consequently suppresses the expression of HSP70.