胸水高迁移率族蛋白B1测定的临床意义研究

Study on the clinical significance of HMGB1 determination in pleural fluids

目的探讨胸水高迁移率族蛋白B1水平检测对胸水性质鉴别的临床意义。方法采用酶联免疫吸附试验对26份漏出液、37份炎症性渗出液和42份肿瘤性渗出液胸水标本进行HMGB1检测,并与白细胞计数作相关性分析。结果炎症性渗出液和肿瘤性渗出液的HMGB1水平均显著高于漏出液(P<0.01),而炎症性渗出液的HMGB1水平明显高于肿瘤性渗出液(P<0.01);胸水HMGB1对渗出液鉴别的灵敏度为89%时,其特异性为84%;渗出液中HMGB1水平与白细胞计数间具有正相关性(r=0.487,P<0.01)。结论胸水HMGB1检测对于胸水性质的鉴别具有较好的临床意义。

Objective To investigate HMGB1 level in pleural fluids and its clinical significance.Methods HMGB1 levels in 105 pleural fluids（26 transudates,37 inflammatory exudates and 42 malignant exudates） were determinated by ELISA,and was statistically analysed for its correlativity with white cell count.Results HMGB1 level was significantly elevated in the exudates as compared with the transudates（P 0.01）.HMGB1 level in the inflammatory exudates was higher than in malignant exudates（P 0.01）.The sensitivity and specificity of HMGB1 level in pleural fluids were 89% and 84% for exudate differentiation,respectively.There was significant positive correlativity between HMGB1 level and white cell count in exudates（r=0.487,P 0.01）.Conclusion The determination of HMGB1 concentration may be used for clinical differentiation of pleural fluid types.