锌试剂与Pepsin作用的UV光谱研究

SPECTROPHOTOMETRIC STUDY ON THE INTERACTION BETWEEN O｛2[α-(2-HYDROXY-5-SULPHOPHENYLAZO) BENZYLIDENE]HYDRAZINO｝BENZOIC ACID AND PEPSIN

本文采用 UV光谱法研究锌试剂 ( ZCN)与 Pepsin(胃蛋白酶 ,Porcine mucosa)相互作用的基本条件及反应机理。研究了不同条件对该反应体系的影响 ,确定了最佳 p H2 .1 5为该配合物反应的酸度条件。测得其配合物的最大吸收峰值 λmax为 560 nm和 596nm,其标准曲线质量浓度在 ( 0～ 30 0 ) ug/m L范围内呈线性关系 ,服从朗白比耳定律。最低捡出限为 0 .8mg/L。研究了温度、时间对体系的影响 。

The interaction of O｛2[α (2 Hydroxy-5-Sulphophenylazo) benzylidene] hydrazino｝benzoic acid with Pepsin in pH.2.15 solution was studied by UV spectrophotometric method.The maximum absorption wavelength of the complex λ max is 460nm.The liner range of the standard curve is～300ug/mL for pepsin,while the detection limit is 0.8mg/L.The basic condition of temperature and time of the reaction were investigated.