阿片类药物对肝癌小鼠细胞免疫功能及IL-2和TNF-α的影响

Effects of Cellular Immunity,IL-2 and TNF-α from Opioid Drugs on Hepatic Cancer Mice

目的探讨不同的阿片类药物对肝癌小鼠细胞免疫功能及IL-2和TNF-α的影响。方法采用细胞接种的方法建立H22肝癌小鼠动物模型,随机分成5组,每组10只,流式细胞仪技术检测肝癌小鼠T淋巴细胞亚群中CD3+、CD4+、CD8+、CD4+/CD8+数值,双抗体夹心法(ELISA)检测小鼠血清IL-2和TNF-α数值。结果与0.9%氯化钠溶液组相比哌替啶组中肝癌小鼠T淋巴细胞亚群中CD3+、CD4+、CD8+、CD4+/CD8+没有明显变化(P>0.05),吗啡组、芬太尼组和舒芬太尼组均明显降低(P<0.05);与0.9%氯化钠溶液组比较哌替啶组小鼠血清IL-2含量明显升高(P<0.05),血清TNF-α含量无明显差别(P>0.05),与0.9%氯化钠溶液组比较吗啡组、芬太尼组、舒芬太尼组血清IL-2和TNF-α均明显降低(P<0.05)。结论吗啡、芬太尼、舒芬太尼对肝癌小鼠T淋巴细胞亚群和IL-2、TNF-α明显抑制。哌替啶对肝癌小鼠T淋巴细胞亚群抑制不明显,能提高血中IL-2含量,对TNF-α水平无明显抑制。

Objective To study the effects of cellular immunity,IL-2 and TNF-a caused by Opioid drugs on hepatic cancer mice. Methods The models of H22 hepatic cancer mice were established by the cell inoculation method;and the animals were randomly divided into 5 groups. ] 0 rats in each group were picked up randomly to detect of lymphocyte subsets CD3 ＋ , CD4 ＋ , CD8 ＋ and CD4 ＋/CD8 ＋ counts. The number of IL 2 and TNF-a were detected by Enzyme-linked Immunosorbent Assay（ELISA）. Results Compared with the group Saline,the numbers of CD3＋ ,CD4＋ ,CD8＋ and the ratios of CD4＋/CD8＋ showed no sig- nificant different in group Pethidine（P〈0.05）, but decreased significantly in group Morphine, group Fen tanyl and group Sufentanil（P〈0.05）. Compared with group Saline, the numbers of IL-2 increased significantly in group Pethidine（P%0.05）;and the numbers of TNF-a showed no significant difference（P〉 0.05） ;but the numbers of IL-2 and TNF-adecreased significantly in group Morphine, group Fentanyl and group Sufentanil （ P 〈 0.05 ）. Conclusion Morphine, Fentanyl and Sufentanil can suppress the immune function,significantly decrease IL-2 and TNF-a of hepatic cancer in mice. Pethidine suppress the immune function unobviously,and the numbers of TNF-a has no significant difference, but significantly increase the numbers of IL 2.