河南省淮滨县中华按蚊的抗药性研究

Resistance of Anopheles sinensis to Insecticides in Huaibin County of Henan Province

用成蚊接触筒法和幼虫浸渍法测定河南省淮滨县中华按蚊子1代Ⅳ龄幼虫和3～5日龄成蚊对敌敌畏、溴氰菊酯和残杀威的抗药性，用色度法测定幼虫和成蚊的乙酰胆碱酯酶（AChE）在加入上述3种杀虫剂前后的活性，计算抑制率。结果显示，0．05％溴氰菊酯（0．0178g／m2）药纸测定中华按蚊成蚊的死亡率为51．2％（〈80％），判断为抗性群体（R）；中华按蚊幼虫对敌敌畏、溴氰菊酯和残杀威等3种杀虫剂的半数致死浓度（EC50）分别为2．76、320．85和754．32mg／L50 0．005mmol／L敌敌畏、溴氰菊酯和残杀威对幼虫AChE的抑制率分别为77．8％、62．8％和58．9％，对成蚊的抑制率分别为76．1％、62．2％和58．2％，对幼虫和成虫的AChE活性均有显著的抑制作用（均P〈0．01）。

Anopheles sinensis aduhs were captured from Huaibin County and raised in laboratory. Larvae dipping method and the WHO tubes were used to determine the resistance of F1 IV-stage larvae and 3-5 day-old adult mosquitoes to dimethyl dichloroviny phosphate （DDVP）, deltamethrin, and arprocarb. The activity of acetylcholine esterase （ACHE） of larvae and adult mosquitoes was measured by colorimetric method before and after the respective addition of the 3 insecticides. The results showed that the mortality of adult mosquitoes in 0.05% deltamethrin testing tube （0.017 8 g/m2） was 51.2%, which was less than 80% and judged as resistant group. The LCso of larvae to DDVP, deltamethrin, and arprocarb were 2.76, 320.85, 754.32 mg/L, respectively. The inhibition rates of 0.005 mmol/L DDVP, deltamethrin, and arprocarb to AChE activity were 77.8%, 62.8%, and 58.9% in larvae, while 76.1%, 62.2%, and 58.22% in adults, respectively, showing a significant inhibition on the AChE activity of An. sinensis （P〈O.O1）.