一种旋毛虫肌幼虫囊包标本的单染制作方法

A Single Staining Method for Specimen of Trichinella spiralis Encapsulated Larvae

本研究采用醋酸明矾卡红染色液对旋毛虫肌幼虫囊包标本进行染色。为观察不同染色时间对制片效果的影响,分别采用4种染色方法:①染色20 h后,分色;②染色2.5 h,不分色;③染色20 h,不分色;④染色40 h后,分色。结果显示,染色方法 1制作的标本效果最佳。囊包、囊内幼虫和周围肌纤维色泽差别较大,易于观察。囊壁内外两层结构可清晰辨别,囊内幼虫典型。

Trichinella spiralis encapsulated larvae speciemens were stained with acetate alum carmine staining solution. Four procedures were compared： （1） speciemen stained for 20 h, and faded;（2） speciemen stained for 2.5 h without fading; （3） speciemen stained for 20 h without fading; （4） speciemen stained for 20 h, and faded. The results showed that the first method is optimal, which clearly distinguishes the capsula, encapsulated larva and muscle cells.