趋化因子CXCL11在急性坏死性胰腺炎大鼠胰腺组织的表达及其作用

Expression and role of chemokine CXCLll in pancreas of rats with acute necrotizing pancreatitis

目的观察趋化因子CXCL11在急性坏死性胰腺炎（ANP）病程中的动态变化，探讨其在ANP发病过程中的作用。方法48只SD大鼠按数字表法随机分为对照组和ANP组，每组24只。采用4％牛黄胆酸钠（1ml／kg体重）逆行胰胆管注射方法制备ANP大鼠模型。术后1、3、6、12h处死大鼠，留取标本。检测血清淀粉酶活性，胰腺组织行常规病理检查并评分，免疫组化法检测胰腺组织CXCL11表达，定量PCR法检测胰腺组织CXCL11mRNA表达，酶联免疫吸附试验法检测血清CXCL11水平。结果ANP组大鼠血清淀粉酶活性较对照组显著升高[6h时为（6153±355）U／L比（185±32）U／L，P〈0．05]；胰腺病理损伤明显，病理评分较对照组显著增加[6h时为（9．00±0．63）分比（0．33±0．12）分，P〈0．05]；胰腺组织CXCL11mRNA及蛋白表达较对照组显著增强（6h时为3．13±0．43比0．99±0．24，2．76±0．27比0．33±0．12，P值均〈0．05）；血清CXCL11水平较对照组明显升高[6h时为（112．1±14．2）ng／L比（56．8±4．3）ng／L，P〈0．05]。结论CXCL11是急性胰腺炎早期的炎症介质，参与了大鼠ANP的发病过程。

Objective To investigate the dynamic expressions of CXCLll and its role in the pathogenesis of acute necrotizing pancreatitis （ANP）. Methods Forty-eight SD rats were randomly divided into control group and ANP group, with 24 rats in each group. ANP model was induced by retrograde injection of 4% sodium taurocholate （1 ml/kg body weight ） into the biliary and pancreatic duct. The rats were sacrificed at 1, 3, 6, 12 hours. Serum level of amylase was determined, pathological changes in pancreatic tissue were routinely observed and scored. The expression of CXCL11 mRNA and proteon in pancreas was measured by fluorescence quantitative polymerase chain reaction and immunohistochemical method. The serum levels of CXCLll were measured by enzyme-linked immunoadsorbent assay. Results The serum levels of amylase in ANP rats were significantly higher than those in control group [ （6153 ±355）U/L vs （185 ±32）U/L at 6 h, P 〈0.05 1, pathological changes in pancreatre tisues were more significant in ANP rats, and the pathological score was significandy higher than that in control group [ （9.00 ±0.63） vs （0.33 ±0.12） points at 6 h, P 〈 0.05 ] ; the expressions of CXCL11 mRNA and protein in pancreatic tissue were significantly increased than those in control group （3.13 ±0.43 vs 0.99 ±0.24, 2.76 ±0.27 vs 0.33 ±0.12 at 6 h, P 〈0.05）. The serum level of CXCL11 was significantly higher than that in control group [ （ 112.1 ± 14.2） ng/L vs （56.8 ±4.3 ）ng/L at 6 h, P 〈0.05 ）]. Conclusions CXCLll is an early inflammatory mediator in acute panereatitis, and involved in the pathogenesis of ANP in rats.