摘要
目的观察早期自然流产患者蜕膜组织中Granulysin、Perforin的表达及其在介导细胞凋亡中的相关性。方法采用免疫组织化学SP法检测30例早期自然流产(流产组)和30例人工流产(对照组)蜕膜组织中Granulysin、Perforin的表达情况,应用TUNEL法检测两组细胞凋亡指数。结果①流产组中Granulysin及Perforin的阳性表达均显著高于对照组,差异有统计学意义(P<0.05)。②Granulysin与Perforin在流产组蜕膜组织中的阳性表达呈正相关(r=0.592,P<0.05)。③流产组的平均细胞凋亡指数[(7.39±3.13)%]高于对照组[(3.45±1.35)%],差异有统计学意义(P<0.05)。④流产组中,Granulysin、Perforin阳性表达组的平均细胞凋亡指数[(8.48±3.08)%,(8.15±3.02)%]均高于阴性表达组[(4.83±1.17)%,(5.00±1.93)%],差异有统计学意义(P<0.05)。结论 Granulysin、Perforin与早期自然流产有关,且两者在早期自然流产的发病过程中有协同作用。
Objective: To observe the expression of Granulysin and Perforin in decidua in patients with early spontaneous abortion(ESA) and investigate their relationship in the course of mediated apoptosis.Methods: The expression of Granulysin and Perforin were detected by immunohistochemistry in decidua of ESA(abortion group,n=30) and normal first trimester pregnant women(control group,n=30),and the apoptotic index was detected by TUNEL.Results: ①The Granulysin-positive expression and Perforin-positive expression were both higher in the abortion group compared with that in the control group,and the difference was statistically significant(P0.05).②The expression of Granulysin in decidua of ESA had significiant positive correlation with that of Perforin(r=0.592,P0.05).③The average apoptotic index [(7.39±3.13)%] of the abortion group was higher than that of the control group [(3.45±1.35)%],and the difference was statistically significant(P0.05).④In the abortion group,the average apoptotic index of Granulysin-positive group [(8.48±3.08)%] and Perforin-positive group [(8.15±3.02)%] were both higher than that of Granulysin-negative group [(4.83±1.17)%] and Perforin-negative group [(5.00±1.93)%],and the difference was statistically significant(P0.05).Conclusion: Granulysin and Perforin may play a certain synergy in the pathogenesis of early spontaneous abortion.
出处
《中国优生与遗传杂志》
2012年第12期22-24,46,共4页
Chinese Journal of Birth Health & Heredity