荧光定量PCR对不育症患者解脲支原体和沙眼衣原体感染情况的研究

Research of infectious status of Ureaplasm urealyticum and Chlamydia trachomatis in fertility by fluorescence quantitative PCR

目的用实时荧光定量PCR(FQ-PCR)定量检测不孕不育患者解脲支原体(UU)、沙眼衣原体(CT)基因,观察UU、CT感染情况与不孕不育的关系,探讨实时荧光定量PCR在不孕不育患者诊断和治疗中作用。方法采用FQ-PCR技术检测标本402份。结果 UU阳性率是32.8%,其中男性UU阳性率是21.4%,女性UU阳性率是44.3%。CT阳性率是16.2%,其中男性CT率是11.4%,女性CT率是20.9%。UU感染率在男性、女性患者有极显著性差异(P<0.01),而CT感染率在性别之间有显著性差异(P>0.05)。结论解脲支原体(UU)、沙眼衣原体(CT)是引起男女不育的主要原因之一,实时荧光定量PCR检测UU、CT基因具有操作简单、反应时间短、重复性好、结果客观准确、敏感性和特异性好等优点,其结果对临床诊断、治疗有一定指导意义。

Objective： To detect quantitatively the gene of Ureaplasm urealyticum（UU） and Chlamydia trachomatis（CT）In fertility by technology of fluorescence quantitative polymerase chain reaction（FQ-PCR）,to understand further infections condition of UU and CT,and to provide clinical treatment evidence to the disease.Methods： Detection 402 speciments by technology of FQ-PCR.Results： the positive of UU was 32.8% of all these;male positive was 21.4%,and female was 44.3%.the positive of CT was 16.2% of all these;male posiaive was 11.4%,and female was 20.9%.Infectious rates of UU、 CT had obvious statistics difference between males and females.Conclusion： The fluorescence quantitative PCR assays showed advantages which were mainpulated simply,time—saving,more extra,more sensitive and more spectific in detecting CT and UU.FQ-PCR had directive meaning for dagnosis and treatmeat.